Theory, Estimation, Experiment, and Data. Hrabchak C, Flynn L, Woodhouse KA. Pharmacol Pharm. Home > 2022 > Junho > 22 > Uncategorized > dextran hydrodynamic radius. 43-1, 115-147. Carbohydrate Research, 160, 205-214. The PubMed wordmark and PubMed logo are registered trademarks of the U.S. Department of Health and Human Services (HHS). Ther. By using this website, you agree to our Accessibility The optical image and histological analysis confirmed that the micropores on the skin were recovered over time. Microporation was performed 2, 5, and 10 times on each sample. ; Rani, K.Y. Indo AM J Pharm. However, in vitro investigations are very few. The calculated dextran molecular weights are corroborated by those tabulated data by the manufacturer with a relative error lower than 10%, being the exception dextrans 580 and 2000kDa with the highest relative error. 4, 10, and 20K) was measured using the Transdermal diffusion cell system. Determination of polymer structure by Gel Permeation Chromatography. Journal Argentine Chemical Society 100 (2013) 25-34. International Journal of Biological Macromolecules, 23, 135-141. In addition, we evaluated the regeneration of SC after microporation. Kasaai, Mohammad R. (2007). Although, in literature there is much information on hydrodynamic measurements from determinations viscosity; very few of them evaluate the situation at different temperatures. Using the polymer standards, a plot of the ln [] vs ln Mw usually gives a straight line. ; Dutt, N.V.K. DLS tests were conducted at detection angle of 90 (standard) and 173 (backscatter) to measure protein hydrodynamic radius, while protein charge was examined in phase analysis light scattering (PALS) mode at detection angle of 15 (forward). Unperturbed dimensions and the theta temperature of dextran in ethylene glycol solutions. The column was eluted with bidestilled water a flow rate of 0.8 ml/min, injection 5L, back pressure 200psi. Food Funct. A similar article of dextran/water [35] clearly demonstrating the dependence of Mark-Houwink parameters with temperature. Part of 2012;27(3):20212. Dear Sir. Alan F Rawle , Can you help me in calculating the size of dextran 4 (molecular weight= 4000) and dextran 10 (molecular weight 10,000)? Th Dextran theta temperature between 43.14 and 44.44C for all the range of molecular weights explains the deviation at 45 and 50C [36, 37]. WebDiffusivity of 2000 kDa Dextran was significantly lower for both hydrogel formulations, but was insensitive to collagen concentration, likely due to a larger hydrodynamic diameter ( dh ~ 70 nm) on order of the collagen mesh size. Epub 2021 Feb 9. These results suggest that fibroblasts proliferate and migrate under the SC, and regenerate the skin. Figure4 shows the histological analysis of micropores in human cadaver skin. Formul. The mostly used commercial product/ polysaccharide is dextran, and it is produced from bacterium Leuconostoc mesenteroides, with an overall degree of branching of about 5% [1-3][1]. Intrinsic viscosity-molecular weight relationship for chitosan. NCI CPTC Antibody Characterization Program. The radius of gyration is typically calculated from static scattering measurements, while the hydro-dynamic radius (or equivalent sphere radius) is deter-mined by dynamic light scattering or other diffusion measurements. Here we have the hydrodynamic radius estimate for the 3kDa dextran and 1.5 kDa dextran by the Zetasizer calculator, thanks to Alan. Alan F Rawle, t He, L.; Niemeyer, B. Advances in Carbohydrate Analysis, 1, 63-144. B 113, 8020-8025. After microporation, the TEWL values of the human cadaver skin increased from 11.30.6 to 33.01.0, 10.71.5 to 45.31.5, and 10.70.6 to 61.02.0g/m2/h when the microporation was performed 2, 5, and 10 times, respectively. Table 2 compares the properties of nanoparticles coated with one or other polymer. WebTry out PMC Labs and tell us what you think. However, considering the trauma and unethical practices associated with animal experiments, in vitro studies with human cadaver skin offers an ethical and viable alternative. Gel permeation chromatography of dextrans in parenteral solutions: Calibration procedure development and method validation. Polysaccharide hydrogels for modified release formulations. The a value is higher than calculated in this work, because these calculations were performed with number average molecular weight (Mn) which rearranges the value of a and forces him to enter into unbranched or linear molecules (a > 0.5), on the other hand, if molecular weights (Mw) ware used to value would be less than 0.5. This phenomenon is observed in the case of hyperbranched biopolymers evidencing an increase of a with temperature. ; Fran, H.P. Effect of temperature on the intrinsic viscosity and conformation of chitosan in dilute HCl solution. One day before the experiment, the skin was stabilized in the skin culture medium. Biomaterials Research The dextran was chosen as another branched polysaccharide which, like the others was suspected to show hyperbranched behavior. Radiofrequency ablation of malignant liver tumors. Masuelli, Martin Alberto. Journal of Applied Polymer Science, 27, 795-810. European Polymer Journal, 36, 2143-2146. Microporation applications for enhancing drug delivery. 2023 BioMed Central Ltd unless otherwise stated. Pig skins (Micropig Franz cell membrane) were supplied by Medi Kinetics Co., Ltd. (Pyeongtaek, Korea). Journal of Colloid Science, 31, 308-328. At the same temperature, the hydrodynamic radius and the intrinsic viscosity are higher for different molecular weights, while these properties decrease with an increase of temperature at the same molecular weight (Figure 1). On the molecular weight distribution of dextran T-500. A few percent of R (13) glucosidic linkages provides side chains which appear to be short. Hydrodynamic radii were measured using Dynamic Light Scattering (DLS) in a Malvern Zetasizer NanoZS DLS (Malvern Instruments, Malvern, UK) at high scattering angle (173) to minimise the effects of dust and supramolecular contamination (NIBS, 173). sharing sensitive information, make sure youre on a federal Typically, the hydrodynamic diameter of the micelles is about 100 nm and the critical micelle concentration is about 10 mg/L. Guner, A.; Kibarer, G. (2001). Dextran consists of -D glucose units with a majority of (16) glucosidic linkages between them. Table 1 shows some values of dextrans Mark-Houwink parameters, from literature consulted, where all data are based on the logarithmic plot of intrinsic viscosity [] and molecular weight Mw. Harding, S. E., & Simpkin, N. J. Privacy This low polydispersity realizes that the Mark-Houwink equation is forced at a given Mw regardless of the hyperbranched nature of the dextran molecule. Analytical Chemistry. Not for use in diagnostic procedures. Figure1 shows a schematic illustration of transdermal delivery after the application of the microporation device. Saudi Pharm J. Disclaimer. Ortega, A.; Garca de la Torre, J. Biotechnology Progress, 19, 544-548. Int. WebThe micelles produced at pH 4.6 have a spherical shape and their size is dependent on the Maillard reaction: reaction time, molar ratio of casein to dextran, and molecular weight of dextran used. Dextran produced from Weissella cibaria JAG8 was purified and characterized. All branched polysaccharide types could be described approximately by the hyperbranching theory. Smith, David R.; Raymonda, John W. (1972). To improve drug permeability, micropore ablation using RF is performed by passing an alternating electrical current through the skin at a frequency higher than 100kHz. ; Bataille, B. Polymer molecular weight distribution. PMC This result could reflect the presence of some aggregated species within the lysozyme sample solution. Dextran (DX) is one type of EPS synthesized by LAB in the presence of sucrose (R g), radius of hydrodynamic (R h), polymer conformation parameter ( = R g /R h), intrinsic viscosity ([]), Mark-Houwink coefficient () and branching degree of DXs of RPYs prepared under different conditions. SEC of mono-carboxymethyl cellulose (CMC) in a wide range of pH, MarkHouwink constants. The Hydrodynamic Radii of Macromolecules and Their Effect on Red Blood Cell Aggregation. Webdextran fraction and the final product depend on changes in the hydrodynamic volume of the molecules after substitution. Boudry I, Blanck O, Cruz C, Blanck M, Vallet V, Bazire A, Capt A, Josse D, Lallement G. Percutaneous penetration and absorption of parathion using human and pig skin models in vitro and human skin grafted onto nude mouse skin model in vivo. Durand, A. Structural characterization of a new dextran with a low degree of branching produced by Leuconostoc mesenteroides FT045B dextransucrase. The most widely applied coatings for FDA-approved SPIONs are dextran and carboxydextran. European Polymer Journal, 42, 1086-1092. Analyzing the values of the hydrodynamic properties of dextran in aqueous solution, it can be seen in Table 7, that all vary with the temperature. (2008). Journal of Chemical Education, 80(9), 1036-1038. Treatment of acne scars by fractional bipolar radiofrequency energy. Barrier integrity was examined using Tewameter to confirm the poration of human cadaver skin using a microporation device. WebThe invention relates to novel fluorescent dyes with multiple negatively charged groups in their ionized form which are 9-aminoacridines or 1-aminopyrene shaving of one of the fol The hydrodynamic radius of 2-NBDG glucose was too small to measure. In addition, the permeation rate was higher in pig skin than in human skin. The first term on the left-hand side of equation 2 (i/c) has a linear relation as a function of the concentration (c in g/cm3), where is the intrinsic viscosity [] is given by (c0) [42, 43]. For simplicity, macromolecules are treated as rigid molecules for a hydrodynamic study. The slope is a value and intercept is equal to the ln kMH value [47]. There is a deviation from linearity after 580kDa [69, 70]. Cecopieri-Gmez, Martha L.; Palacios-Alquisira, Joaqun (2005). H&E stained images were analyzed using Image J software. (2003). The increased TEWL values which were proportional to the number of microspores indicate SC disruption and formation of microchannels in the skin. Unable to load your collection due to an error, Unable to load your delegates due to an error. For a given polymer-solvent pair, the theta condition is satisfied at a certain temperature, called the theta () temperature or theta point. Interaction Parameter (); Expansion Factor (); Steric Hindrance Factor (); and Shielding Function F(); for the System PEAOrganic Solvents by Intrinsic Viscosity Measurements. ; Ortega, A.; Garcia de la Torre, J.; Harding, S.E. 4h after microporation, the sizes of the micropore decreased slightly. DOI: 10.12691/jpbpc-1-1-3, Received September 18, 2013; Revised October 23, 2013; Accepted November 22, 2013. It has been found that, when applied to water treatment, it can effectively act on refractory organic matter in wastewater through thermal decomposition and water molecular action to produce free Pharmaceut Sci Tech. (1969). Fixable: Fixable via Lysine. Takara, Andres; Acosta, Adolfo; Masuelli, Martin A. Lucifer yellow (LY) and FITC-labelled dextran stock solutions were prepared in Ringers-HEPES buffer (150 mM NaCl, 3.4 mM CaCl 2, 1.2 mM MgCl 2, 5.2 mM KCl, 0.5 mM NaHCO 3, 2.8 mM glucose, and 10 mM HEPES) and frozen at 20C. European Polymer Journal, 43, 1744-1753. Journal of Physical Chemistry, 58(11), 953-957. Coviello, T.; Matricardi, P.; Marianecci, C.; Alhaique, F. (2007). At 20C data "a" value obtained in this work is intermediate to those given by references [1, 37] while the value of kM-H is lower. Microporations were performed 2, 5, and 10 times and the skin damage was confirmed using the TEWL measurement. Degrees of long-chain branching in dextrans. The characteristic Rg/Rh value for a globular protein is ~0.775, which means that Rg is smaller than Rh. 2004;25(9):155967. Synthesis of dextran by dextransucrase treated, sucrose-supplemented skimmed milk revealed the promising potential of dextran as a food additive. Kasaai, M. R.; Arul, J.; Charlet, G. (2000). The https:// ensures that you are connecting to the Equivalent Radii and Ratios of Radii from Solution Properties as Indicators of Macromolecular Conformation, Shape, and Flexibility. Solution properties of branched dextran. Pharm Res. European Journal of Pharmaceutics and Biopharmaceutics, 68, 319-329. We observe that this maximum radius is below In a poor solvent which shows a positive heat of mixing, segments of a polymer molecule attract each other in solution more strongly than attract the surrounding solvent molecules.